FERMENT PROPHYLACTIC help prevent tooth decay

FERMENT PROPHYLACTIC help prevent tooth decay

The present invention relates to prophylactic ferments that promote the prevention of dental caries through bacteriological properties competitive vis-à-vis any flora. Cariogenic properties and food for their industrial use. These ferments are particularly suited to food and diet, for example associated with lactic acid bacteria for the manufacture of yoghurt and other products made of fermented milk, and the pharmaceutical industry, for example in the form of freeze-dried tablets.

The tooth decay is an infectious disease transmitted widespread whose main responsibility is Streptococcus mutans, a bacterium that can destroy the enamel and dentin by releasing acids in the mouth during the degradation of carbohydrates. According to the nomenclature in force (Bergey's Manual 8th edition, 1984 and 1986) the species S. mutans is actually formed by eight-called serotypes A to H: S. mutans itself (serotypes c, e, f), S. rattus (serotype a), S. cricetus (serotype b) and S. sobrinus (serotypes d, g, h). Cariogenic species of this group (S. mutans c, f, S. sobrinus d and g) are particularly characterized by their ability to colonize the soft tissues by means of specific adhesins, to colonize the hard tissue by extra-cellular polysaccharide (dextran or glucans) in Coaggregation with other oral bacteria to rapidly form acids from sugars and survive on very low pH. Other oral bacteria can enhance that power in cariogenic caries lesions start or established, especially other species of genus Streptococcus species and genera Actinomyces (A. naeslundii and A. odontolyticus) and Lactobacillus (casei and L. fermentum ). Cavities leads to progressive and irreversible destruction of the tooth infringement that, untreated, can cause localized infections or widespread. The current preventive techniques based on an oral hygiene correct, rational consumption of carbohydrates and the use of hygiene products and Dental fluoro. Although effective these methods require individual active cooperation is not always observed, especially among children, so the bunt is still a major public health problem. The device according to the invention is based on the principle of competition interbactérienne and ecological balance in the oral flora: it can help prevent tooth decay by adding exogenous repeated at the plate

dental, small amounts of virulent non enablers prophylactic likely to oppose the colonization of dental surfaces by any cariogenic flora dominated by pathogenic strains of S. Indeed mutans, plaque early to recolonize the enamel in subjects free of active caries is dominated by bacteria type

5 Gram positive cocci, immobile, aérotolérants and / or capsules, in particular S. sanguis (serotype 1: S. gordoπii, serotype 2: S. mitior or S. oralis), S. mitis (serotypes 1 and 2) and Stomatococcus mucilaginosus, a non-cariogenic encapsulated commensal of the oral flora. These species are found in varying proportions depending on the individual, location and age of the deposits. The species within the constitution of

10 prophylactic ferments are selected:

- Among all non-virulent strains of bacteria compatible with maintaining the integrity oral

- Among the early colonizers of the plate above in particular S. mitis (ATCC 15 909, ATCC 15 912), S. salivarius (ATCC 9758, ATCC 13 419, ATCC 27

15 945), and Stomatococcus mucilaginosus (ATCC 25 296, CCM 2417, NCTC 10 663),

- Among the non-virulent strains of S. mutans, L casei (in paticulier L casei subsp. rhamnosus ATCC 7469, 20 012 DSM, DSM 20 021, 20 022 DSM, DSM 20 023, 20 245 DSM, DSM 20 247), and L fermentum,

- Among the non-virulent strains of L. acidophilus (ATCC 4356), L. crispatus (ATCC 33 20 820, VR 3199), L. gasseri (DSM 20 243) and L salivarius (L. salivarius salivarius

ATCC 1 1 741, L salivarius salicinus ATCC 1 1 742) who have the double advantage of presenting a tropism for the oral environment and belonging to the group of lactobacilli thermophilic directly usable as lactic.

- Among all mutants avirulent derivatives of all species mentioned above, 5 - among all avirulent mutants derived from other bacterial species and introducing new characters they can compete with any ecological flora oral cariogenic, such strains used in the dairy industry (such as Lactobacillus bulgaricus and Streptococcus thermophilus) and amended to introduce desphénomènes grip and coagrégation specific 0 (pili, fimbriae, adhesins) and / or non-specific (glycocalyx, capsules, poiysaccharides extra-cellular type glucan / dextran or fructans / levani) in the mouth.

This list is by no means exhaustive and may change depending on progress in understanding the oral flora and updating of cash 5 Taxonomy current or future. The seeds are formed prophylaxis of one or more of several species mentioned above by Associated

SHEET OF REPLACEMENT

example in reports of 1 / 1, and can be kept frozen in liquid nitrogen or dry based industrial objectives sought. It should be noted that these prophylactic ferments are also very different from the flora associated with periodontal disease, which does not exclude a possible additional prophylactic effect in the context of periodontal disease.

Yoghurt, khéfirs and other products made of fermented milk is an excellent medium for this type of prevention as usual lactic used to manufacture precisely belong to the genera Lactobacillus and Streptococcus. These products therefore perfectly suited to the culture and survival fermenting concurrently prophylactic allowing an influx of germs live immediately competitive at the plaque and a contribution decalcium that promotes remineralization fronts caries demineralization. In this case, the prophylactic ferments can be used in addition to the usual lactic ferments at a concentration optimal given the biological, technical and gastronomic, eg in the range of final concentrations ranging from 1 / 10 ** 1 / 101 0 compared to lactic ferments. They are used either as coferments if they support the manufacturing processes and in particular the heat (for example the association L. salivarius, L. acidophilus and L casei subsp. Rhamnosus in proportion 1/1/1) either by simple addition as brewing at the end of manufacture (for example the association L. salivarius, S. salivarius and S. mucilaginosus in proportion 1 / 1 / 1). These are also ferments prophylactic interesting freeze-dried and distributed by example by 1 to 2 million units in the form of pharmaceutical tablets to suck slowly with a suitable vehicle such as a carbohydrate not fermentable. Indeed, the oral environment is an environment conducive to the retention (coagrégation bacterial) and the growth of bacteria lyophilized (temperature, pH. Humidity, the presence of nutrients and growth factors, bacterial synergy, etc.).. These examples of prophylactic use of fermentation are not exhaustive, and can be used in any other form whatsoever, particularly associated with all chemical compounds known to have a preventive effect against tooth decay, such compounds fluorinated or carbohydrates non-fermentable.

The device according to the invention is especially written for dietetic industries, especially pharmaceuticals and food because of the widespread consumption / widespread yoghurt and other products made of fermented milk, especially among children for whom the prevention of dental caries is a major concern.